Pfu DNA polymerase (500 U) kit (Not Taq polymerase)
10X Cloned Pfu Buffer. 10x Pfu Reaction Buffer (containing 2 mM MgSO. The reaction mixture was incubated at 72°C for 30 minutes. 10x Pfu Buffer. The reaction products were resolved in an agarose gel containing ethidium bromide and visualized. ).